Exogenous induction of the Escherichia coli hexose phosphate transport system defined by uhp-lac operon fusions
نویسندگان
چکیده
منابع مشابه
Compartmentation in the induction of the hexose-6-phosphate transport system of Escherichia coli.
The induction of the hexose-6-phosphate transport system was investigated. Glucose-6-phosphate (G6P) at concentrations as low as 10(-4)m was able to induce this system in wild-type cells, as well as in mutants lacking phosphoglucose isomerase or G6P dehydrogenase. Growth in the presence of fructose-6-phosphate (F6P) induced the system only if the cells contained phosphoglucose isomerase. Furthe...
متن کاملRole of the phosphotransferase system in Escherichia coli strains deficient in hexose phosphate transport.
In Escherichia coli, glucose 6-phosphate is transported via the Uhp system which is inducible by glucose 6-phosphate. We showed that, in a uhp-deficient strain, glucose 6-phosphate was dephosphorylated in the periplasm and that the resulting glucose was subsequently transported into the cells via the phosphotransferase system. The uptake of glucose generated from glucose 6-phosphate allowed the...
متن کاملTranscription of the lac operon of Escherichia coli.
The effects of induction and of transient and catabolite repression on lac-specific RNA in cells of Escherichia coli were investigated. The rate of Zac-specific RNA synthesis increases linearly for 3 min after induction and then becomes constant. The accumulation of RNA complementary to the promoter proximal tat 2 gene begins before accumulation of RNA complementary to the promoter distal lac Y...
متن کاملDeterminants of bistability in induction of the Escherichia coli lac operon.
The authors have developed a mathematical model of regulation of expression of the Escherichia coli lac operon, and have investigated bistability in its steady-state induction behaviour in the absence of external glucose. Numerical analysis of equations describing regulation by artificial inducers revealed two natural bistability parameters that can be used to control the range of inducer conce...
متن کاملTransposition of the lac region of Escherichia coli. I. Inversion of the lac operon and transduction of lac by phi80.
The power of recombinant DNA technology stems from the ability it provides to create novel DNA joints; this method uses enzymes to join unrelated DNA fragments in vitro. For example, two novel DNA joints are created when a gene is cloned in vitro onto a small plasmid vector. Transposition and illegitimate recombination also create novel DNA joints. Accordingly, when harnessed properly by geneti...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1981
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.148.1.203-209.1981